目的分析贵州施秉太子参不同栽培类型、不同繁殖方式和不同生长时间生物量与次生代谢产物的动态变化规律,为施秉太子参品种选育、药材合理采收提供指导。方法以3个栽培类型、2种繁殖材料为主要研究对象,定期记录植株生物量的动态变化,HPLC测定块根太子参环肽B含量。结果随着生长时间的变化,无性繁殖的栽培样本XZ-1、XZ-2、XZ-3分别在7月初、6月下旬和7月上旬达到最高地下生物量(9.86g/株、3.61g/株和10.40g/株),太子参环肽B含量仅XZ-1在7月上旬达到药典规定(0.0213%)。在采收期,XZ-1和XZ-3的地下生物量显著高于XZ-2,而XZ-1和XZ-2的太子参环肽B含量显著高于XZ-3。同一栽培类型有性繁殖XZ-1Y的生物量显著低于无性繁殖XZ-1,但太子参环肽B含量则显著高于XZ-1。结论综合考虑生物量和太子参环肽B含量,太子参XZ-1的品质较好,属于值得推广的栽培类型,也适宜作为优良品种的选育对象,其最佳采收期应为植株倒苗后10d左右。
Abstract
OBJECTIVE To study the dynamic accumulation of biomass and secondary metabolites from different cultivation forms,reproduced by different modes in different growth periods of Pesudostellaria heterophylla in Shibing district,Guizhou province,in order to provide guidance for variety breeding and rational harvest method of P.heterophylla. METHODS Taking three cultivation forms and two kinds of breeding materials as the main research subjects,the dynamic accumulation of biomass was periodically recorded with growth period change,and the contents of pseudostellarin B in the roots of P.heterophylla were analyzed by HPLC. RESULTS The underground biomass in cultivation form of vegetative propagation XZ-1,XZ-2 and XZ-3 reached the highest levels of 9.86,3.61 and 10.40 g穚lant-1 in early July,late June and the beginning of July,respectively. The content of pseudostellarin B reached the criterion of Chinese Pharmacopoeia only in XZ-1 in early July,which was 0.021 3%. In the harvest time,the contents of biomass in XZ-1 and XZ-3 were significantly higher than that in XZ-2,while the contents of pseudostellarin B in XZ-1 and XZ-2 were significantly higher than that in XZ-3. The content of biomass in sexual reproduction material,XZ-1Y,was significantly lower than that in the vegetative propagation one,XZ-1 of the same cultivation form,but the content of pseudostellarin B in XZ-1Y was significantly higher than that in XZ-1. CONCLUSION According to the comprehensive consideration of the contents of biomass and secondary metabolites,the XZ-1 of P. heterophylla has better quality,and it is worth popularizing and being used for variety breeding. The optimal harvest time of Pesudostellaria heterophylla is about 10 days after the plant is withered.
关键词
太子参 /
栽培类型 /
生物量 /
太子参环肽B
{{custom_keyword}} /
Key words
Pesudostellaria heterophylla /
cultivation form /
biomass /
pseudostellarin B
{{custom_keyword}} /
中图分类号:
R284
{{custom_clc.code}}
({{custom_clc.text}})
{{custom_sec.title}}
{{custom_sec.title}}
{{custom_sec.content}}
参考文献
[1] Ch.P(2010)VolⅠ(中国药典 2010年版.一部).2010:62-63.[2] LONG Q J.Research and education on harvesting and primary processing of Chinese medicinal materials.Chin J Inf Tradit Chin Med(中国中医药信息杂志),2011,18(11):3-4.[3] WANG M,SONG J P,HAN Y,et al.Study on the content and dynamic variation of Pseudostellarin B in Pesudostellaria heterophylla. J Chin Med Mater(中药材),2010,33(8): 1225-1227.[4] AN K,HE J,WAN Z M,et al.Determination and multivariate statistical analysis of amino acid in Pseudostellaria heterophylla from different producing areas.Nat Prod Res Dev(天然产物研究与开发),2012(05):594-598.[5] LIN M,ZHENG J,YANG L,et al.Chemical compositions of Pseudostellaria heterophylla (Miq.) Pax cultivated in different regions.Food Sci(食品科学),2012,33(2):204-207.[6] XIAO C H,ZHOU T,JIANG W K,et al.Comparative analysis of main agronomic traits and interrelationships in cultivation provenances of Pesudostellaria heterphylla from Guizhou province.China J Chin Mater Med (中国中药杂志),2013,38(6):812-816.[7] PENG H S,LIU W Z,HU Z H.Advances in studies on biology and chemical constituents in Radix Pseudostellariae.Chin Tradit Herbal Drugs(中草药),2008,39(3):470-473.[8] HUANG L Q,GUO L P.Secondary metabolites accumulating and geoherbs formation under envirom ental stress.China J Chin Mater Med (中国中药杂志),2007,32(4): 277-280.[9] QIN M J,XU Y B,HUANG W Z,et al.Quality assay of Radix Pseudostellariae collected from different regions.Res Pract Chin Med(现代中药研究与实践),2005,19(5):29-32. QIONG J,FANG K H,LIU X H,et al.Study on the dynamic change of Polysaccharide content in Radix Pseudostellariae.Lishizhen Med Mater Med Res(时珍国医国药),2009,20 (9): 2238-2240.
{{custom_fnGroup.title_cn}}
脚注
{{custom_fn.content}}
基金
施秉中药材产业科技合作专项计划(施科合专项(2010)02号、(2012)04号);贵州省教育厅自然科学项目(黔教科(2011)031号);贵阳市科技计划项目(筑科合同大-3号);贵阳中医学院研究生教育创新计划项目(ZYYCX11033)
{{custom_fund}}